Composite

Part:BBa_K3273006:Design

Designed by: Bruno Batista, Fernando de Souza, Isabelle Taira, Lucas Boldrini   Group: iGEM19_Sao_Carlos-Brazil   (2019-10-14)


AGA2_Linker_4D


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 280
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The 4D peptide has repetitive sequences, making it very difficult to construct directly by "de novo" synthesis. Therefore, we break it into two parts, in order to link them by Gibson assembly


Source

This part was developed combining parts found in the registry

References